Examining serum amyloid P component microheterogeneity using capillary isoelectric focusing and MALDI-MS

Examining serum amyloid P component microheterogeneity using capillary isoelectric focusing and MALDI-MS.

Serum amyloid P component (SAP) is a glycoprotein of interest due to its presence in amyloid plaque formations. As with most glycoproteins, SAP can possibly vary greatly in its isoforms, which can be an important factor toward understanding the role of SAP. Interestingly, previous characterizations suggest varying degrees of microheterogeneity, some of which are in conflict. In this work, we pr...

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...

Optimizing Conditions for Capillary Isoelectric Focusing Separations Using

1136 Serum Amyloid P Component Binds Fibronectin

Amyloid P component (AP) 1 is a glycoprotein found in all forms of amyloid deposit regardless of the chemical nature of the fibril protein (1-6). It is indistinguishable from a normal plasma protein, serum amyloid P component (SAP), in terms of antigenicity, molecular weight, polypeptide subunit composition, appearance in the electron microscope, and the partial amino acid sequences available h...

Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection.

CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...